Congratulations to Gianmarco Raddi, PhD on the publication of ‘Mosquito cellular immunity at single-cell resolution’ in Science. The study discusses the discovery of new types of mosquito immune cells, including a rare cell type that could be involved in limiting malaria infection, as well as identifying molecular pathways implicated in controlling the malaria parasite. Findings from this atlas of cells offer opportunities for uncovering novel ways to break the chain of malaria transmission from mosquito to human. The atlas will also be a valuable resource for researchers trying to understand and control other mosquito-borne diseases such as Dengue or Zika.

We are proud to have helped this research in a small way by providing the Sanger Institute with vivoPHIX™ to enable the fixation, stabilisation and single cell preparation of hemocytes for scRNA-seq (10x Genomics).

“We have carried out the first ever large scale survey of the mosquito immune system, and using single cell sequencing technology we found immune cell types and cell states that had never been seen before.’ said Gianmarco Raddi, PhD – lead author of the study. “We also looked at mosquitoes that were infected with the Plasmodium parasite and for the first time were able to study their immune response in molecular detail, and identify which cells and pathways were involved.”

View the publication in Science online here:

https://science.sciencemag.org/content/369/6507/1128

vivoPHIX™ is a patented RNA stabilisation solution that has unique properties to enable cell dissociation. The reagent is capable of fixing cells, preserve RNA and inactivate viruses for further analysis all the way down to a single cell level, such as scRNA-seq and 10x Genomics. For more information about vivoPHIX™, click here.

If you are interested in using vivoPHIX™ for single cell work, please contact us at rnassist@rapidlabs.co.uk