Introducing virusPHIX: The SARS-CoV-2 Virus Transport Medium

  • Inactivates SARS-CoV-2 and other viruses & bacteria
  • Stabilises SARS-CoV-2 RNA for at least 14 days at 20°C
  • Suitable for swab and saliva testing
  • Contains no toxic chemicals and safe for home use
  • Optimised in partnership with UK Lighthouse labs for compatibility with automated pipetting robots in high throughput labs (Hamilton, Ortho & Tecan)
  • Manufactured in the UK by Rapid Labs

SARS-CoV-2 Inactivation Results published by Public Health England

PHE evaluated the reduction in virus titre SARS-CoV-2 spiked samples (tissue culture fluid) and presence of SARS-CoV-2 over a series of cell passages.

Triplicate samples of tissue culture fluid containing 5 %%(v/ foetal calf serum, spiked with SARS CoV 2 were treated with virus PHIX ™ for indicated contact time/s or mock treated in triplicate with an equivalent volume of PBS Purified samples were immediately titrated on Vero E 6 cells to establish virus titre Reduction in virus titre following treatment is given as the difference between the mean log 10 TCID 50 /ml for treated conditions and the PBS control (Test 1). In parallel, purified samples were seeded onto Vero E6 monolayers to amplify any remaining virus over the course of up to four serial passages This test is qualitative and reports either the presence or absence of virus amplification (Test 2).

View the report here: https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/937439/HCM-CoV2-034-v2_virusPHIX_TCF__5_.pdf

Virus Inactivation

virusPHIX™ has inactivated all tested viruses and bacteria, including SARS-CoV-2, Dengue, Zika, mTb, Vaccinia, Duck Hepatitis B (a surrogate for Human Hepatitis B), Feline Immunodeficiency Virus (a HIV surrogate), Vaccinia and many others. For the full list of viruses & bacteria inactivated by virusPHIX™, please contact us at rnassist@rapidlabs.co.uk.

Long term stability of RNA viruses

Independent studies have shown stability of SARS-CoV-2 RNA for 33 days at room temperature and 12 month stability of Hepatitis C Virus RNA at 24°C:

Independent study by Qnostics UK demonstrated that virusPHIX+™ stabilises SARS-CoV-2 virus RNA for at least 33 days at 20°C. Sputum samples spiked with SARS-CoV-2 RNA were analysed over 33 days, and the Ct value was compared with virusPHIX+™ treated samples and control (no stabiliser added)

Fig 1. Independent study by Qnostics UK demonstrated that virusPHIX+™ stabilises SARS-CoV-2 virus RNA for at least 33 days at 20°C. Sputum samples spiked with SARS-CoV-2 RNA were analysed over 33 days, and the Ct value was compared with virusPHIX+™ treated samples and control (no stabiliser added)

Fig 2. Additional independent study by Qnostics UK demonstrates 12 month stability of Hepatitis C virus when treated with virusPHIX™ and stored at room temperature. The same 2 HCV strains were stable for 6 months when stored at -80°C without virusPHIX™.

Mosquito host cells were incubated for 5 days before staining with anti-Dengue antibody. No Dengue virus presence was detected. Read the paper here.

The difference in working in a Cat 3 (CL3) vs Cat 2 (CL2) lab can have huge implications from a financial and logistical perspective in addition to the stringent health and safety measures put in place.

Dengue samples were treated with vivoPHIX™ (the precursor to virusPHIX™) and shipped from East Africa to Paris via Dubai at ambient temperature.

The study published in Science discusses the discovery of new types of mosquito immune cells, including a rare cell type that could be involved in limiting malaria infection, as well as identifying molecular pathways implicated in controlling the malaria parasite.
The Sanger Institute used vivoPHIX™ to enable the fixation, stabilisation and single cell preparation of hemocytes for scRNA-seq (10x Genomics).

vivoPHIX™ is the sister product to virusPHIX™ which offers the same stabilisation and inactivation properties, with the additional properties of enabling single cell analysis.

To determine the ability of vivoPHIX™ (a sister product of virusPHIX, developed for single cell analysis work) to inhibit ZIKV in mosquito tissue sample, ZIKV-infected mosquito midguts were dissected at 6 dpi. Mosquito midguts were then chopped into 3-4 small pieces then transfer into vivoPHIX™ . Samples were then treated at 4 degree Celsius for 30 minutes before infectious virus titration by focus forming assay. All of the mosquito in the untreated group were infected with >50,000 FFU/mosquito midgut, while no viable virus particles could be detected in the vivoPHIX™ treated particles.