Aeromonas Agar Base

/Aeromonas Agar Base

Aeromonas Agar Base

Cat. Number: CM-AAB190 Categories: , ,

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Description

Use and Description:

For the selective isolation of Aeromonas hydrophila from clinical and environmental samples. Aeromonas Agar Base is based on the formulation of Ryan – which is the modification of XLD Medium that has been designed to improve the count and isolation of Aeromonas in clinical and environmental samples. The medium is better than any other medium in the detection of Aeromonas in water, bottled water and food samples.

Proteose peptone is the nitrogen, vitamin, minerals and amino acids source which are essential for growth. L-Lysine and L-arginine provides nitrogen, sulphur and trace elements. Yeast extract needs to be the source of B group vitamins. Carbohydrates are lactose, sorbitol and xylose. Sodium thiosulphate provides sulphur and ferric ammonium citrate is the indicator of H2S production. H2S positive colonies have black centre. Mixed indicators change their colour too yellow when acid is formed.

Composition per litre:

Peptones ……………………………… 5.0 g

Sodium Thiosulphate …………..10.7 g

Sodium Chloride ……………………5.0 g

Xylose ………………………….………3.75 g

Sorbitol ………………………….………3.0 g

Inositol ………………………..….…….2.5 g

Lactose …………………….……….….1.5 g

L-Lysine………………………………….3.5 g

L-Arginine …………………..…………2.0 g

Yeast extract……………..………..…3.0 g

Ferric Citrate …………………..…… 0.8 g

Bile Salts ………………………….……3.0 g

Thymol blue – Bromthymol blue 0.04-0.04

Agar ………………………………….12.5 g

 

Final pH of the ready to use medium: 8.0±0.2 at 25.0 °C

 

Medium preparation:

Add 28.1 grams of dehydrated culture medium to 500ml litre of distilled water. Boil for 1 minute. Do not overheat or autoclave it.

 

Quality specifications:

1. The powder is homogeneous, free flowing and beige.

2. Ready to use medium: greenish-blue

 

Microbiological response after 22-24 hours at 30-35 °C:

Organism Growth Colony / Colour
Aeromonas hydrophila ATCC 7966 Good Green with  black centre
Pseudomonas aeruginosa ATCC 27853 Good Blue-green
Pseudomonas aeruginosa ATCC 9270 Good Blue-green
E. coli ATCC 25922 Inhibited

 

Storage:

Store the sealed bottle containing the dehydrated medium at 25 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the colour has changed from the original light beige colour.