Amies Transport Medium (modified) with Charcoal (1%)

/Amies Transport Medium (modified) with Charcoal (1%)

Amies Transport Medium (modified) with Charcoal (1%)

Cat. Number: CM-ATMC048 Categories: , ,

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Description

Use and description:

For transport and maintenance of microbiological samples at the moment with charcoal is used for collecting, transporting and preserving microbiological specimens. It is formulated to maintain the viability of microorganisms without significant increase in growth, being non-nutritive, phosphate buffered and semi-solid. Amies developed his formula (1967) with charcoal upon proving that Neisseria gonorrhoeae increased its survival rate when charcoal swabs were used. Amies solved the problem of charcoal removal from the swabs by incorporating charcoal into the formulation. Amies Transport Medium is recommended for throat, vaginal, and wound samples. In the formulation, Charcoal neutralises fatty acids that are toxic to microorganisms. The Chloride salts supply essential electrolytes for transport and osmotic balance. Phosphates act as a buffer system. Sodium thioglycollate suppresses oxidative changes and provides a reduced environment.

 

Composition per litre g/l:

Activated Charcoal…………………………………..10.00

Monopotassium Phosphate……………………… 0.20

Sodium Chloride………………………………………. 3.00

Calcium Chloride……………………………………… 0.10

Disodium Phosphate……………………………….. 1.10

Magnesium Chloride……………………………….. 0.10

Sodium Thioglicollate………………………………. 1.00

Agar………………………………………………………… 7.50

Potassium Chloride…………………………………. 0.20

 

Final pH of the ready to use medium: 7.3 +/- 0.2 at 25°C

 

Medium preparation:

Add 28.1 grams of dehydrated culture medium to 500ml litre of distilled water.

Boil for 1 minute. Do not overheat. Do not autoclave.

 

Quality specifications:

1. The powder is homogeneous, free flowing and black

2. Ready to use medium: black

 

Microbiological response after 72 hours at 4°C to 25°C:

Organism Recovery at 4°C Recovery at 25°C
Neisseria gonorrhoeaeATCC 19424 ≥50% ≥50%
Brucella abortusATCC 4315 ≥50% ≥50%
Shigella flexneriATCC 12022 ≥50% ≥50%
Salmonella typhiATCC 6539 ≥50% ≥50%