Azide Dextrose Broth

/Azide Dextrose Broth

Azide Dextrose Broth

Cat. Number: CM-ADB299 Categories: , ,

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Description

Use and description:

Azide Dextrose Broth is used in the detection of fecal streptococci in water, sewage, and milk. Principles of the Procedure Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, and Beef Extract are the carbon, nitrogen, and vitamin sources used for general growth requirements in Azide Dextrose Broth. Dextrose is a fermentable carbohydrate. Sodium Chloride maintains the osmotic balance of the medium. Sodium Azide inhibits cytochrome oxidase in Gram-negative bacteria.

 

Composition per litre:

Peptones ……………………………… 15.0 g

Dextrose ……………………….……..….7.5 g

Meat Extract ……………………………4.5 g

Sodium Chloride ……………..……… .7.5g

Sodium Azide …..………………..……..0.2g

 

Final pH of the ready to use medium: 7.2 ± 0.2 at 25°C

 

Medium preparation:

Add 34.7 grams of dehydrated culture medium to 1 litre of distilled water. Mix thoroughly to completely dissolve the medium. Autoclave it for 15 minutes at 121°C.

 

Quality specifications:

1. The powder is homogeneous, free flowing cream to light beige

2. Ready to use medium: brilliant to clear, yellow to gold

 

Microbiological response at 37 °C after 18-48 hours incubation:

Organism Result
Escherichia coli ATCC 25922 inhibited
Enterococcus faecalis ATCC 29212 growth
Staphylococcus aures ATCC 25923 inhibited

 

Storage:

Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the colour has changed from the original light beige colour.