Blood Agar Base No.2

/Blood Agar Base No.2

Blood Agar Base No.2

Cat. Number: CM-BAB120 Categories: , ,

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Description

Use and description:

This agar is used for the isolation of fastidious bacteria. It allows to identify the haemolytic pattern and formation of pigments. With chocolated blood it gives good recovery of Haemophilus spp. With antibiotic addition it can be used for selective isolation of Haemophilus and campylobacter and Brucella and streptococci.

 

Composition per litre:

Tryptose……………………………………17.0 g

Yeast extract…………………………..…..5.0 g

Sodium chloride………………………….5.0 g

Agar………………………………………….12.0 g

 

Final pH of the ready to use medium: 7.4 ± 0.2

 

Medium preparation:

Add 39 grams of dehydrated culture medium to 1 litre of distilled water. Mix thoroughly. Heat with frequent agitation and boil for 1 min to completely dissolve. Autoclave for 15 min at 121 ⁰C. Cool to 47 ⁰C. Aseptically add 5-10% of sterile, defibrinated blood. Pour into sterile Petri dishes. To prepare chocolate blood agar the blood should be added at 80 ⁰C with intensive mixing.

 

Quality specifications:

Dehydrated medium: homogeneous, light beige fine powder.

Ready to use medium: opaque and cherry red when supplemented with blood.

 

Microbiological response at 37 ⁰C after 18-24 hours:

Organism Result Haemolysis
Staphylococcus aureus ATCC 25923 Growth Beta
Streptococcus pneumoniae ATCC 6305 Growth Alpha
Streptococcus pyogenes ATCC 19615 Growth Beta

 

Storage:

Dehydrated medium should be stored between 10 to 25 ⁰C. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or if the colour has changed from the original.