Brilliant Green Agar Modified

/Brilliant Green Agar Modified

Brilliant Green Agar Modified

Cat. Number: CM-BGA167 Categories: , ,

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Description

Use and description:

Selective medium for the isolation of salmonellae. The modification was to increase the selectivity of the medium by increasing the brilliant green concentration. To increase selectivity further it is recommended to include 1 g/l of sodium sulfacetomide and 250 mg/l of sodium mandellate. Salmonella colonies are red, pinkish or white but they are always surrounded by a red halo, which shows the inactivity of lactose or sucrose. The growth of lactose and/or sucrose fermenting bacteria are partially inhibited but in the case of growth they produce yellow-green colonies surrounded by a yellow halo. Proteus and Pseudomonas are inhibited, they produce small, red colonies.

 

Composition per litre:

Peptone………………………………..….10.0 g

Meat extract………………………………5.0 g

Yeast extract………………………………3.0 g

Lactose………………………………….….10.0 g

Sucrose……………………………………..10.0 g

Disodium phosphate……………………1.0 g

Sodium phosphate…………….………..0.6 g

Phenol red………………………………90.0 mg

Brilliant green………………..…………5.0 mg

Agar………………………………..………..12.0 g

 

Final pH of the ready to use medium: 6.9 ± 0.2

 

Medium preparation:

Add 52.0 grams of dehydrated culture medium to 1 litre of distilled water. Heat gently with occasional agitation and bring just to the boil to dissolve the medium completely. DO NOT AUTOCLAVE THIS MEDIUM.

 

Quality specifications:

Dehydrated medium: homogeneous, pinkish fine powder.

Ready to use medium: orange-brown agar, very slightly opalescent.

 

Microbiological response:

Organism Result
Salmonella typhimurium ATCC 14028 Good growth
Escherichia coli ATCC 25922 Negative, inhibited
Proteus vulgaris ATCC 13315 Negative, inhibited

 

Storage:

Dehydrated medium should be stored between 10 to 25°C. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or if the colour has changed from the original.