Chromogenic Candida

/Chromogenic Candida

Chromogenic Candida

Cat. Number: CM-CM01 Categories: , ,

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Description

Use and description:

This media is recommended for isolation and differentiation of Candida spp. It contains chloramphenicol to inhibit a wide range of bacteria, and chromogenic Mix for biochemical differentiation of Candida.

 

Composition per litre:

Agar………………………. 15.00g

Peptones………………… 12.00g

D-Glucose……………….. 15.70g

Chromogenic mix……… 2.30g

Chloramphenicol………. 0.45g

 

Final pH of the ready to use medium: 5.5 ± 0.2

 

Medium preparation:

Add 45.45 grams of culture medium to 1 litre of distilled water and allow to soak for 10 minutes. Swirl to mix and heat up until completely dissolved. Sterilise at 110°C at 10 minutes. Allow to cool to 47°C, and then pour plates.

 

Quality specifications:

  1. The powder is homogenous, free flowing and clear amber
  2. Ready to use medium: straw, clear to slightly opalescent agar

 

Microbiological response after 48 hours incubation at 35-37°C:

Organism Result
Candida albicans ATCC 10231 Emerald, white metallic shine
Candida dublinesis NCPF 3949 Dark green, matte
Candida glabrata ATCC 1031 White pinkish, shiny
Candida krusei ATCC 142403 Light pink
Candida tropicalis ATCC 750 Deep magenta, some strains with small white outer ring
E.coli ATCC 25922 No growth

 

Storage:

Store the sealed bottle containing the dehydrated medium at 25 – 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the colour has changed from the original clear amber colour.