Cooked Meat Medium

/Cooked Meat Medium

Cooked Meat Medium

Cat. Number: CM-CMM214 Categories: , ,

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Description

Use and description:

Cooked Meat Medium is a well-established medium for the cultivation of anaerobic and aerobic organisms. Bacteria hydrolyzing glucose rapidly acidify the medium and produce gas without digesting the meat. Proteolytic strains break the meat down to amino acids causing a blackening and a putrid smell due to of sulphur compounds. The tube of medium is incubated with the cap loose and no seal is required. Aerobes grow at the top whilst anaerobic species grow deeper in the medium. In the prepared medium, two phases can be defined: solid particles in the bottom and a transparent amber liquid over them. Meat particles provide good circumstances for forming microenvironment that help anaerobic growth deeper in the medium. Aerobs grow at the top of the medium. The following supplements can be added to increase selectivity, glucose (5 g/L), hemin (5 mg/L), vitamins (1 mg/L) and yeast extract (5 g/L).

 

Composition per litre:

Beef heart………………………………….98.0 g

Peptones……………………………………20.0 g

Glucose…………………………………..…..2.0 g

Sodium chloride………………………….5.0 g

 

Final pH of the ready to use medium: 7.2 ± 0.2

 

Medium preparation:

Add 125.0 grams of dehydrated media to 1 litre of distilled water. Mix thoroughly. Distribute into tubes in 10.0 ml volumes. Autoclave for 15 min at 121°C. In the case of aerobic culture do not seal and tighten caps. Incubation time 7 days at 35°C. In the case of anaerobic culture incubate sealed tube with tightened cap. Incubation time 21 days at 35°C.

 

Quality specifications:

Dehydrated medium: brown pellets.

Ready to use medium: in a test tube appears as medium clear amber supernatant over insoluble granules.

 

Microbiological response:

Organism Result
Escherichia coli ATCC 25922 Growth
Clostridium perfringens ATCC 13124 Growth
Clostridium sporogenes ATCC 11437 Growth
Staphylococcus aureus ATCC 25923 Growth

 

Storage:

Dehydrated medium should be stored between 10 to 25°C. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or if the colour has changed from the original.