Kligler Iron Agar

/Kligler Iron Agar

Kligler Iron Agar

Cat. Number: CM-KIA194 Categories: , ,

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Description

Use and description:

For the differentiation and identification of Enterobacteriaceae based on the fermentation of two sugars and the H2S production. Sugar fermentation is indicated by turning phenol red indicator to yellow. H2S production results in the formation of iron sulphide blackening in the middle and upper portion of the slant. In the case of gas formation there are bubbles or cracks in the medium. Fermentation of glucose only, is followed by reversion in pH on the slope, from initial acidity to final alkalinity (red colour), but not in the anaerobic conditions of the butt, which remains acid (yellow). Fermentation of lactose as well as glucose, produces acidity (yellow) in both slope and butt. Liberation of sulphide results in the formation of iron sulphide (blackening of either slope or butt).

 

Composition per litre:

Sodium chloride……………………….….…5.000 g

Agar………………………………………….….14.000 g

Lactose…………………………………………10.000 g

Peptone………………………………….……22.000 g

Glucose…………………………………..……..1.000 g

Ferric ammonium citrate….……………0.500 g

Sodium thiosulphate……………………..0.500 g

Phenol red………………………………….……0.03 g

Yeast extract……………………………..…..3.000 g

Meat extract…………………………….……3.000 g

 

Final pH of the ready to use medium: 7.4 ± 0.2

 

Medium preparation:

Add 59.0 grams of dehydrated culture medium to 1 litre of distilled water.

Heat with repeated stirring and boil for one minute to dissolve completely.

Distribute into tubes and autoclave for 15 minutes at 121°C.

After autoclaving, slant tubes to prepare deep butts.

 

Quality specifications:

Dehydrated medium: homogeneous, fine, light beige powder.

Ready to use medium: clear and red to hazy.

 

Microbiological response after 18-24 hours at 35°C:

Organism Slope Butt Gas H2S
Shigella sonnei Red Yellow
Shigella dysenteriae Red Yellow
Salmonella typhi Red Yellow +
Enterobacter Red Yellow +
Klebsiella Yellow Yellow +
Escherichia coli Yellow Yellow +
Morganella Red Yellow variable
Yersinia Red Yellow variable
Citrobacter freundii Yellow Yellow + +
Proteus vulgaris Red Yellow +
Alcaligenes faecalis Red Red

 

Storage:

Dehydrated medium should be stored between 10 to 25°C. Once opened, keep the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the colour has changed from the original.