Description
Use and description:
A medium for the detection, isolation and enumeration yeasts and moulds. The addition of lactic acid suppresses bacterial growth.
Composition per litre:
Malt extract…………………………….29.0 g
Peptone…………………………………….5.0 g
Agar…………………………………………15.0 g
Final pH of the ready to use medium: 5.4 ± 0.2
Medium preparation:
Add 49.0 grams of dehydrated culture medium to 1 litre of distilled water, allow to soak for 10 minutes, swirl to mix then sterilise for 10 minutes at 115°C. Do not overheat because the agar will not harden. Pour into sterile Petri dishes or distribute into sterile tubes.
Quality specifications:
Dehydrated medium: homogeneous, light beige fine powder.
Ready to use medium: very slightly opalescent, amber coloured agar.
Microbiological response:
| Organism | Result |
| Candida albicans ATCC 10231 | Good growth |
| Aspergillus niger ATCC 16404 | Good growth |
Storage:
Dehydrated medium should be stored between 10 to 25°C. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the colour has changed from the original.
500g wrapped pots