Maximal Recovery Diluent

/Maximal Recovery Diluent

Maximal Recovery Diluent

Cat. Number: CM-MRD029 Categories: , ,

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Description

Use and description:

For the transportation and recovery of microorganisms acc. ISO/DIS 6649. This solution protects organism by peptone and physiological saline osmotic support.

 

Composition per litre:

Sodium Chloride………………..…………….8.5 g

Peptone..…………..…………………………..1.0 g

 

Final pH of the ready to use medium: 7.0±0.2

 

Medium preparation:

Add 9.5 grams of dehydrated culture medium to 1 litre of distilled water. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave for 15 min at 121°C. To 10 g test samples add 90 ml of sterile MRD and blend it. Make 10x serial dilutions from the macerate and add 1 ml of it to the chosen medium on 45-50°C. Let it solidify and incubate.

 

Quality specifications:

Dehydrated medium: homogeneous, white fine powder.

Ready to use medium: colourless clear solution.

 

Storage:

Dehydrated medium should be stored between 10 to 25°C. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or if the colour has changed from the original.

500g wrapped pots