Milk Plate Count Agar

/Milk Plate Count Agar

Milk Plate Count Agar

Cat. Number: CM-MPCA061 Categories: , ,

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Description

Use and description:

This medium is Plate Count Agar APHA with antibiotic free skimmed milk recommended by BSI, give standard results in dairy products and to count colony forming units per ml of sample.

 

Composition per litre:

Yeast extract……………………………….2.5 g

Tryptone………………………………..……5.0 g

Glucose……………………………………….1.0 g

Agar………………………………………..…15.0 g

Skimmed milk………………………………1.0 g

 

Final pH of the ready to use medium: 7.0 +/- 0.2

 

Medium preparation:

Add 24.5 grams of dehydrated culture medium to 1 litre of distilled water. Bring to the boil with frequent stirring to dissolve. Dispense into tubes and sterilise by autoclaving for 15 min at 121°C.

 

Quality specifications:

Dehydrated medium: homogeneous, cream coloured fine powder.

Ready to use medium: white, opalescent agar.

 

Microbiological response:

Organism Result
Staphylococcus aureus ATCC 25923 Good growth

 

Storage:

Dehydrated medium should be stored between 10 to 25°C. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the colour has changed from the original.

500g wrapped pots