Motilití-Indol-Urea Base (MIU)

/Motilití-Indol-Urea Base (MIU)

Motilití-Indol-Urea Base (MIU)

Cat. Number: CM-MIM111 Categories: , ,

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Description

Use and description:

Semi-solid medium to detect microbiological motility together with urease activity and indol production.

 

Composition per litre:

Peptones……………………………………….15.000 g

Yeast extract……………………………………3.000 g

D-glucose……………………….…………….…1.000 g

Disodium-H-PO4………………………….….2.300 g

Sodium-diH-PO4…..……………………….. 2.200 g

Sodium chloride…………………………….. 2.000 g

Phenol red……………..……………………… 0.012 g

Agar …………………………………………….… 2.000 g

 

Final pH of the ready to use medium: 6.8 ± 0.2

 

Medium preparation:

Add 25.5 grams of dehydrated culture medium to 1 litre of distilled water until evenly dispersed. Heat with repeated stirring until boil and dissolve completely. Autoclave for 15 min at 121°C. Cool down at 50°C. Add 50 ml sterile 40% Urea solution. Note: Urea is heat sensitive. Do not sterilize the solution by autoclaving. Mix well and dispense into the final containers. To detect indol production, add 4-5 drops of Kovacs´ reagent to the cultures after incubation.

 

Quality specifications:

Dehydrated medium: homogeneous, light orange coloured, fine powder.

Ready to use medium: clear and orange semisolid agar, slightly opalescence

 

Microbiological response:

 

Organism Result / motility Indol/Urease
Escherichia coli ATCC 25922 Growth / + – / +
Proteus vulgaris ATCC 13351 Growth / + + / +

 

Storage:

Dehydrated medium should be stored between 10 to 25°C. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or if the colour has changed from the original.

500g wrapped pots