MRS Broth

/MRS Broth

MRS Broth

Cat. Number: CM-MRSB177 Categories: , ,

Product

Sku

Name (required)

Country (required)

Company

Email (required)

Message

Description

Use and description:

This broth is used to encourage and help the growth of lactobacilli from food products. The medium inhibits the growth of different cocci and moulds due to the sodium acetate and ammonium citrate content. Tween 80 supports the growth of bacteria that require fatty acids.

 

Composition per litre:

Peptones……………………………….. 10.00 g

Meat extract……………………………. 8.00 g

Yeast extract……………………………. 4.00 g

D-Glucose……………….……………… 20.00 g

Sodium acetate……………………….. 5.00 g

Ammonium citrate………………….. 2.00 g

MgSO4.7H2O………………………….. 0.20 g

MnSO4 .4H2O…………………………. 0.05 g

K2HPO4…………………………………… 2.50 g

 

Final pH of the ready to use medium: 6.2 +/- 0.2 at 25°C

 

Medium preparation:

Add 51.75 grams of dehydrated culture medium to 1 litre of distilled water, add Tween 80 supports the growth of bacteria that require fatty acids allow to soak for 10 minutes, swirl to mix then distribute into containers and sterilise for 15 min at 121°C.

 

Quality specifications:

Dehydrated medium: homogeneous, light cream, a lightly clumped powder. Ready to use medium: amber coloured solution, very slightly opalescent.

 

Microbiological response:

Organism Result
Lactobacillus delbrueckii subs. ATCC 4797 Good growth
Lactobacillus rhamnosus casei ATCC 9595 Good growth

 

Storage:

Dehydrated medium should be stored between 10 to 25°C. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or if the colour has changed from the original.

500g wrapped pots