Nutrient Agar

/Nutrient Agar

Nutrient Agar

Cat. Number: CM-NA073 Categories: , ,

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Description

Use and description:

Medium for the cultivation of a wide variety of non-fastidious microorganisms.

 

Composition per litre:

Peptone……………………………………….5.0 g

Meat extract………………………….…….1.0 g

Yeast extract………………………………..2.0 g

Agar……………………………………………15.0 g

Sodium chloride……………………………5.0 g

 

Final pH of the ready to use medium: 7.3 ± 0.2

 

Medium preparation:

Add 28.0 grams of dehydrated culture medium to 1 litre of distilled water. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes.

 

Quality specifications:

Dehydrated medium: homogeneous, light beige fine powder.

Ready to use medium: beige agar.

 

Microbiological response:

Organism Result
Escherichia coli ATCC 25922 Good growth
Enterococcus faecalis ATCC 19433 Good growth
Pseudomonas aeruginosa ATCC 27853 Good growth

 

Storage:

Dehydrated medium should be stored between 10 to 25°C. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the colour has changed from the original.

500g wrapped pots