Description
Use and description:
Broth for the cultivation of a wide variety of non-fastidious microorganisms.
Composition per litre:
Peptone…………………………………………. 5.0 g
Meat extract……………………….………….1.0 g
Yeast extract…………………………………..2.0 g
Sodiumchloride………………………………5.0 g
Final pH of the ready to use medium: 7.4 ± 0.2
Medium preparation:
Add 13.0 grams of dehydrated culture medium to 1 litre of distilled water. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes.
Quality specifications:
Dehydrated medium: homogeneous, light beige fine powder.
Ready to use medium: beige broth.
Microbiological response:
| Organism | Result |
| Escherichia coli ATCC 25922 | Good growth |
| Enterococcus faecalis ATCC 19433 | Good growth |
| Pseudomonas aeruginosa ATCC 27853 | Good growth |
Storage:
Dehydrated medium should be stored between 10 to 25°C. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the colour has changed from the original.
500g wrapped pots