Plate Count Agar APHA

Description

Use and description:

This medium recommended by USP and APHA is used to count aerobic bacteria in water, milk and other food products.

Composition per litre:

Yeast extract……………………………. 2.5 g

Tryptone……………………………………5.0 g

Glucose……………………………………..1.0 g

Agar…………………………………………15.0 g

Final pH of the ready to use medium: 7.0 ± 0.2

Medium preparation:

Add 23.5 grams of dehydrated culture medium to 1 litre of distilled water. Bring to the boil with frequent stirring to dissolve. Dispense into tubes and sterilise by autoclaving for 15 min at 121°C. Cool to 44-46°C for not more than 3 hours prior to use. Incubate at 32-35°C for 48 hours.

Quality specifications:

Dehydrated medium: homogeneous, cream coloured fine powder.

Ready to use medium: light amber agar, very slightly opalescent.

Microbiological response:

Storage:

Dehydrated medium should be stored between 10 to 25°C. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or if the colour has changed from the original.

500g wrapped pots