Rapport Vassiliadis Broth

/Rapport Vassiliadis Broth

Rapport Vassiliadis Broth

Cat. Number: CM-RVB070 Categories: , ,

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Description

Use and description:

Selective enrichment broth for the detection of Salmonella in food products and in water. The strong concentration of magnesium and the malachite green, the high incubation temperature and the low pH render the medium inhibitor with regard to other bacteria.

 

Composition per litre:

Peptone ..……………………………………………..……4.500 g

Potassium dihydrogen phosphate………………1.260 g

Dipotassium-H-phosphate…………………………0.180 g

Magnesium chloride………………………………..13.600 g

Sodium chloride…………………………………………7.200 g

Malachite green ………………………………………. 0.036 g

 

Final pH of the ready to use medium: 5.2 ± 0.2

 

Medium preparation:

Add 26.8 grams of dehydrated culture medium to 1 litre of distilled water. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 115°C for 15 minutes.

 

Quality specifications:

Dehydrated medium: homogeneous, beige to pale green fine powder.

Ready to use medium: clear and blue.

 

Microbiological response:

Organism Result
Escherichia coli ATCC 25922 Inhibited
Salmonella typhimurium ATCC 14028 Good growth

 

Storage:

Dehydrated medium should be stored between 10 to 25°C. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the colour has changed from the original.

500g wrapped pots