Salmonella

Chromogenic Dehydrated Culture Media

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    Description

    Use and description:

    For the selective differentiation of salmonella spp. from other pathogenic enterobacteriaceae from food and clinical specimens. Gram-positive bacteria are inhibited by bile salts, citrate. Salmonella spp. will grow as magenta colonies, Enterobacter spp. will grow as deep blue colonies.

     

    Composition per liter:

    Peptones………………………………………. 7.0 g

    L-lysine  ………………………………………..0.5 g

    L-cystine ……………………………………….0.1 g

    Beef Extract ……………………………………5.0 g

    Chromogenix mix……………………………0.30 g

    Agar…………………………………………  …12.0 g

    Yeast extract …………………………………..5.0 g

    Xylose ………………………………………….1.75 g

     

    Final pH of the ready to use medium:   7.0 ± 0.2

     

    Medium preparation:

    Add 31,7 grams of dehydrated culture medium to 1 liter of distilled water, and allow to soak for 10 minutes. Swirl to mix, then bring to the boil and autoclave at 121 C for 15 minutes. Dry the surface before incubation.

     

    Quality specifications:

    Dehydrated medium:  homogeneous, beige fine powder.

    Ready to use medium:  straw coloured agar, clear to very slightly opalescent.

     

    Microbiological response:

    OrganismResult
    Enterococcus faecalis ATCC 29212Inhibited
    Escherichia coli ATCC 25922Partially inhibited, deep blue colonies
    Salmonella typhimurium ATCC 14028Growth, magenta colonies
    Enterobacter aerogenes  ATCC 13048Growth, deep blue colonies

     

    Storage:

    Dehydrated medium should be stored between 10 to 25oC. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or if color has changed from the original.